Adaption strategy of up-flow anaerobic sludge blanket reactor on tetracycline stress during tetracycline antibiotic wastewater treatment

Tetracycline antibiotic wastewater with high chemical oxygen demand (COD) concentration and containing bio-refractory compounds was investigated in a lab-scale up-flow anaerobic sludge blanket (UASB) anoxic-oxic (A/O) reactor. Specific methanogenic activity (SMA) determined to reveal the inhibition levels of methane production volatile fatty acid (VFA) accumulation. The results showed that microorganisms can adapt tetracycline or oxytetracycline when antibiotics is less than 400 mg/L. Acetic acid, propanoic n-butyric accounted for more 90% VFA increasing from 50 mg/L 600 During 215 d operation, increased 0 95 mg/L, COD 3,500 6,500 average removal rates reactors were maintained above 90%. UASB reactor achieved 0.32 L/g COD. accumulation observed 47.5 granule diameter 0–300 μm 300–1,500 during operation. Rod-shaped coccus-shaped organisms pores internal channels on surface using scanning electron microscopy. In space, filamentous bacteria constitute rock-steady structure.